Recent developments of magnetic beads for use in nucleic acid purification

J Chromatogr A. 1998 Aug 7;816(1):107-11. doi: 10.1016/s0021-9673(98)00064-8.

Abstract

The performance of Magarose, an agarose-based bead containing a paramagnetic component has been evaluated. The anion exchanger DEAE-Magarose is effective at binding DNA from a crude cell lysate. The plasmid pBluescript was isolated from 1.5 ml Escherichia coli JM109 cell culture, following alkaline lysis yielding 8.2 micrograms high-quality DNA. Under similar binding conditions 21 micrograms of salmon sperm DNA bound to the ion exchangers. The affinity medium oligo-dT Magarose was demonstrated to bind 75 mumol of an oligo-dA probe/g of medium by hybridization. Under similar conditions mRNA could be isolated from a preparation of baby hamster cell total RNA. The magnetic susceptibility of Magarose is very high, facilitating the use of this separation technique for rapid batch chromatographic processes.

MeSH terms

  • Animals
  • Anion Exchange Resins
  • Chromatography, Ion Exchange
  • Cricetinae
  • DNA, Bacterial / isolation & purification
  • Escherichia coli / chemistry
  • Magnetics
  • Male
  • Nucleic Acids / isolation & purification*
  • Oligonucleotides / chemistry
  • Oligonucleotides / isolation & purification
  • Plasmids / isolation & purification
  • RNA, Messenger / isolation & purification
  • Salmon
  • Sepharose
  • Spermatozoa / chemistry

Substances

  • Anion Exchange Resins
  • DNA, Bacterial
  • Nucleic Acids
  • Oligonucleotides
  • RNA, Messenger
  • Sepharose