Identification of tomato Lhc promoter regions necessary for circadian expression

Plant Mol Biol. 1998 Nov 1;38(4):655-62. doi: 10.1023/a:1006094015513.

Abstract

Expression of the light-harvesting complex protein genes (Lhc) is under the control of a circadian clock. To dissect the molecular regulatory components of the circadian clock a promoter deletion analysis of four tomato Lhc genes was performed in transgenic tobacco plants. The important 5'-upstream promoter regions are present at different positions relative to the transcription start site of Lhc b1*1, b1*2, Lhc a3 and Lhc a4. A short sequence of 47 nucleotides is necessary for conferring circadian Lhc mRNA oscillations. Sequence alignment of the specified promoter regions revealed a novel motif 'CAANNNNATC'. This motif is conserved in 5'-upstream regions of clock controlled Lhc genes and overlaps with a sequence relevant in phytochrome mediated gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Circadian Rhythm / genetics*
  • Circadian Rhythm / physiology*
  • DNA, Plant / genetics
  • Gene Expression Regulation, Plant
  • Genes, Plant
  • Photosynthetic Reaction Center Complex Proteins / genetics*
  • Promoter Regions, Genetic*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Plant / genetics
  • RNA, Plant / metabolism
  • Sequence Deletion
  • Sequence Homology, Nucleic Acid
  • Solanum lycopersicum / genetics*
  • Solanum lycopersicum / physiology*

Substances

  • DNA, Plant
  • Photosynthetic Reaction Center Complex Proteins
  • RNA, Messenger
  • RNA, Plant