A comparison of frozen and paraffin sections in dermatofibrosarcoma protuberans

Dermatol Surg. 1998 Sep;24(9):995-8. doi: 10.1111/j.1524-4725.1998.tb04293.x.

Abstract

Background: Dermatofibrosarcoma protuberans (DFSP) is a tumor with a high local reoccurrence rate. Mohs micrographic surgery offers the highest cure rate. However, differentiating minimal residual tumor from normal skin can be difficult during Mohs surgery.

Objective: To clarify the problem of determining when a tumor-free plane had been achieved during Mohs surgery for a DFSP.

Methods: In two patients with DFSPs, we compared frozen and paraffin-embedded sections extending from tumor to normal skin, using both H&E and CD34 stains.

Results: On frozen, but not paraffin-embedded, sections scattered dermal spindle cells were seen in normal skin.

Conclusions: Scattered dermal spindle cells in the dermis of normal skin make it difficult to differentiate minimal residual tumor from normal dermis during Mohs surgery. A biopsy of normal skin can be useful as a control in this setting.

Publication types

  • Comparative Study

MeSH terms

  • Biopsy
  • Dermatofibrosarcoma / pathology*
  • Dermatofibrosarcoma / surgery*
  • Frozen Sections* / methods
  • Humans
  • Mohs Surgery* / methods
  • Paraffin Embedding* / methods
  • Skin Neoplasms / pathology*
  • Skin Neoplasms / surgery*
  • Specimen Handling*