Diagnosis of Plasmodium falciparum malaria using ParaSight F, ICT malaria PF and malaria IgG CELISA assays

Parasite. 1998 Jun;5(2):189-92. doi: 10.1051/parasite/1998052189.

Abstract

A battery of sixty-six blood samples from Senegal was analysed by the ParaSight F test, the ICT Malaria PF and the Malaria IgG CELISA. These three assays detect the histidine rich protein 2 antigen of Plasmodium falciparum. Thick smear microscopy was used as the reference method. Sensitivity, specificity, predictive positive and negative values were respectively 89%, 100%, 100%, 88% for the ICT; 86%, 93%, 94%, 85% for the paraSight and 88%, 87%, 88%, 87% for the Malaria IgG CELISA. The three assays failed to detect two positive samples with P. ovale and P. malariae. Assays were also compared with regard to the expense of equipment and reagents and speed and ease of use. The rapid ICT and ParaSight F test can be performed with minimal training and may be specially useful in areas where P. falciparum is the predominant malaria species, in epidemic malaria regions, and where skilled microscopy is not readily available.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Animals
  • Antibodies, Protozoan / immunology
  • Antigens, Protozoan / blood
  • Antigens, Protozoan / immunology
  • Child
  • Child, Preschool
  • Enzyme-Linked Immunosorbent Assay*
  • Female
  • Humans
  • Immunoglobulin G / immunology
  • Infant
  • Malaria, Falciparum / diagnosis*
  • Male
  • Middle Aged
  • Parasitemia / diagnosis
  • Plasmodium falciparum / chemistry*
  • Plasmodium falciparum / immunology
  • Plasmodium falciparum / isolation & purification
  • Predictive Value of Tests
  • Proteins / analysis*
  • Proteins / immunology
  • Protozoan Proteins / blood*
  • Protozoan Proteins / immunology
  • Sensitivity and Specificity

Substances

  • Antibodies, Protozoan
  • Antigens, Protozoan
  • Immunoglobulin G
  • Proteins
  • Protozoan Proteins
  • histidine-rich proteins