Abstract
Mutant-enriched PCR and reverse dot blot hybridization in microplates were applied for examining K-ras status in stools and tissue samples from patients with pancreatic tumors and chronic pancreatitis. In tissue samples, K-ras mutations were found in 32 of 35 cases of ductal adenocarcinoma, in 5 of 7 periampullary cancers, in 1 cystadenocarcinoma, and in 3 of 5 patients with chronic pancreatitis. In stools, mutated K-ras was seen in 10 of 25 cases of ductal adenocarcinoma, in 1 case of cystadenocarcinoma, and in 2 of 6 cases of chronic pancreatitis. These data indicate that the K-ras status of stool samples may help identify pancreatic carcinoma and persons at risk for cancer development; however, it does not allow discrimination of malignant from nonmalignant diseases.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / chemistry
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Adenocarcinoma / genetics*
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Adenocarcinoma / pathology
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Biomarkers, Tumor / analysis
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CA-19-9 Antigen / analysis
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Carcinoembryonic Antigen / analysis
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Chronic Disease
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DNA / genetics
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DNA / isolation & purification
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DNA, Neoplasm / genetics
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DNA, Neoplasm / isolation & purification
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Feces / chemistry*
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Humans
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Immunoenzyme Techniques
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Oncogene Protein p21(ras) / analysis
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Oncogene Protein p21(ras) / genetics*
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Pancreas / chemistry*
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Pancreatic Ducts*
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Pancreatic Neoplasms / chemistry
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Pancreatic Neoplasms / genetics*
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Pancreatic Neoplasms / pathology
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Pancreatitis / genetics*
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Point Mutation*
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Polymerase Chain Reaction
Substances
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Biomarkers, Tumor
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CA-19-9 Antigen
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Carcinoembryonic Antigen
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DNA, Neoplasm
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DNA
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Oncogene Protein p21(ras)