Abstract
A new immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) based on the recombinant Epstein-Barr virus (EBV) matrix protein was developed. Compared to indirect immunofluorescence for the detection of IgM antibody to the EBV capsid antigen on clinical specimens, the sensitivity and specificity of the new IgM ELISA were 96 and 96%, respectively.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Viral / blood*
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Antigens, Viral / chemistry
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Antigens, Viral / genetics
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Base Sequence
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Capsid Proteins*
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DNA Primers / genetics
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Enzyme-Linked Immunosorbent Assay / methods*
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Enzyme-Linked Immunosorbent Assay / statistics & numerical data
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Epstein-Barr Virus Infections / diagnosis*
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Epstein-Barr Virus Infections / immunology*
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Evaluation Studies as Topic
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Herpesvirus 4, Human / genetics
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Herpesvirus 4, Human / immunology*
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Humans
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Immunoglobulin M / blood*
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Molecular Weight
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology
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Sensitivity and Specificity
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Viral Matrix Proteins / chemistry
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Viral Matrix Proteins / genetics
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Viral Matrix Proteins / immunology*
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Virology / methods
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Virology / statistics & numerical data
Substances
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Antibodies, Viral
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Antigens, Viral
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Capsid Proteins
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DNA Primers
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Epstein-Barr viral capsid antigen
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Immunoglobulin M
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Recombinant Fusion Proteins
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Viral Matrix Proteins