Abstract
Erythroid Krüppel-like factor (EKLF) is necessary for stage-specific expression of the human beta-globin gene. We show that EKLF requires a SWI/SNF-related chromatin remodeling complex, EKLF coactivator-remodeling complex 1 (E-RC1), to generate a DNase I hypersensitive, transcriptionally active beta-globin promoter on chromatin templates in vitro. E-RC1 contains BRG1, BAF170, BAF155, and INI1 (BAF47) homologs of yeast SWI/SNF subunits, as well as a subunit unique to higher eukaryotes, BAF57, which is critical for chromatin remodeling and transcription with EKLF. E-RC1 displays functional selectivity toward transcription factors, since it cannot activate expression of chromatin-assembled HIV-1 templates with the E box-binding protein TFE-3. Thus, a member of the SWI/SNF family acts directly in transcriptional activation and may regulate subsets of genes by selectively interacting with specific DNA-binding proteins.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Binding Sites
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Chromatin*
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Chromosomal Proteins, Non-Histone
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DNA Helicases
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DNA-Binding Proteins / metabolism*
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Drosophila Proteins*
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Gene Expression Regulation*
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Globins / genetics
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HIV-1 / genetics
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Humans
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Kruppel-Like Transcription Factors
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Macromolecular Substances
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Nuclear Proteins / metabolism*
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Promoter Regions, Genetic
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RNA-Binding Proteins*
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Ribonucleoprotein, U1 Small Nuclear / chemistry
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SMARCB1 Protein
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Transcription Factors / chemistry
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Transcription Factors / metabolism*
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Transcription, Genetic
Substances
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Chromatin
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins
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Drosophila Proteins
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Kruppel-Like Transcription Factors
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Macromolecular Substances
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Nuclear Proteins
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RNA-Binding Proteins
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Ribonucleoprotein, U1 Small Nuclear
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SMARCB1 Protein
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SMARCB1 protein, human
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SMARCC1 protein, human
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SMARCC2 protein, human
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Transcription Factors
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erythroid Kruppel-like factor
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snf protein, Drosophila
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Globins
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SMARCA4 protein, human
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DNA Helicases