A strategy for the generation of infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome

J Herold; V Thiel; S G Siddell

doi:10.1007/978-1-4615-5331-1_34

A strategy for the generation of infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome

Adv Exp Med Biol. 1998:440:265-8. doi: 10.1007/978-1-4615-5331-1_34.

Authors

J Herold¹, V Thiel, S G Siddell

Affiliation

¹ Institute for Virology and Immunology, University of Würzburg, Germany.

PMID: 9782291
DOI: 10.1007/978-1-4615-5331-1_34

Abstract

A strategy to generate in vitro transcripts representing infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome is presented. PCR-DNAs were ligated in vitro, resulting in 27 kbp and 22 kbp ligation products. These DNAs can now be transcribed in vitro and the RNAs tested for infectivity and their ability to replicate.

MeSH terms

Coronavirus / genetics*
Coronavirus / pathogenicity
Coronavirus 229E, Human*
DNA, Viral
Genome, Viral*
Humans
RNA, Viral / biosynthesis*
Reverse Transcriptase Polymerase Chain Reaction
Transcription, Genetic

Substances

DNA, Viral
RNA, Viral