Abstract
The 39-kilodalton protein (P39) has previously been shown to be an immunodominant protein in Brucella infections. P39 gene deletion mutants of vaccine strains Brucella abortus S19 and Brucella melitensis Rev.1 were constructed by gene replacement. This deletion did not significantly modify the residual virulence of both vaccine strains in CD-1 mice. CD-1 mice vaccinated with the parent or mutant strains were protected against a virulent challenge. Mutant vaccine strains devoid of P39 could provide a means for differentiating vaccinated from infected animals.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Bacterial / genetics*
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Antigens, Bacterial / immunology*
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Brucella Vaccine / genetics
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Brucella Vaccine / immunology*
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Brucella abortus / genetics
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Brucella abortus / immunology*
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Brucella abortus / pathogenicity
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Brucella melitensis / genetics
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Brucella melitensis / immunology*
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Brucella melitensis / pathogenicity
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Brucellosis / immunology
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Brucellosis / prevention & control*
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Disease Models, Animal
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Female
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Gene Deletion*
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Mice
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Virulence / genetics
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Virulence / immunology
Substances
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Antigens, Bacterial
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Brucella Vaccine
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brucellin