Abstract
DNA immunization has been investigated as a potential immunization strategy against infectious diseases and cancer. To enhance a DNA vaccine's ability to induce CTL response in vivo, we co-administered CD80 and CD86 expression cassettes along with HIV-1 immunogens. This manipulation resulted in a dramatic increase in MHC class I-restricted and CD8+ T-cell-dependent CTL responses in both mice and chimpanzees. This strategy of engineering vaccine producing cells to be more efficient T-cell activators could be an important tool for optimizing antigen-specific T-cell-mediated immune responses in the pursuit of more rationally designed vaccines and immune therapies.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antigen Presentation
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Antigens, CD / biosynthesis
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Antigens, CD / genetics*
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Antigens, CD / immunology*
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B7-1 Antigen / biosynthesis
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B7-1 Antigen / genetics*
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B7-1 Antigen / immunology*
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B7-2 Antigen
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CD8-Positive T-Lymphocytes / immunology
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Cloning, Molecular
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DNA, Complementary / administration & dosage
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DNA, Complementary / genetics
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DNA, Complementary / metabolism
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Epitopes / immunology
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HIV Antigens / genetics
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HIV Antigens / immunology
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HIV-1 / immunology
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Histocompatibility Antigens Class I / immunology
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Lymphocyte Activation / immunology
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Lymphokines / biosynthesis
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Membrane Glycoproteins / biosynthesis
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Membrane Glycoproteins / genetics*
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Membrane Glycoproteins / immunology*
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Pan troglodytes
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T-Lymphocytes, Cytotoxic / immunology
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T-Lymphocytes, Helper-Inducer / immunology
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Vaccines, DNA / genetics*
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Vaccines, DNA / immunology*
Substances
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Antigens, CD
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B7-1 Antigen
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B7-2 Antigen
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Cd86 protein, mouse
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DNA, Complementary
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Epitopes
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HIV Antigens
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Histocompatibility Antigens Class I
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Lymphokines
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Membrane Glycoproteins
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Vaccines, DNA