DNA vaccines with single-chain Fv fused to fragment C of tetanus toxin induce protective immunity against lymphoma and myeloma

Nat Med. 1998 Nov;4(11):1281-6. doi: 10.1038/3266.

Abstract

Vaccination with idiotypic protein protects against B-cell lymphoma, mainly through anti-idiotypic antibody. For use in patients, DNA vaccines containing single-chain Fv derived from tumor provide a convenient alternative vaccine delivery system. However, single-chain Fv sequence alone induces low anti-idiotypic response and poor protection against lymphoma. Fusion of the gene encoding fragment C of tetanus toxin to single-chain Fv substantially promotes the anti-idiotypic response and induces strong protection against B-cell lymphoma. The same fusion design also induces protective immunity against a surface Ig-negative myeloma. These findings indicate that fusion to a pathogen sequence allows a tumor antigen to engage diverse immune mechanisms that suppress growth. This fusion design has the added advantage of overcoming potential tolerance to tumor that may exist in patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cancer Vaccines*
  • Immunoglobulin Fragments*
  • Immunoglobulin M
  • Immunoglobulin Variable Region*
  • Immunoglobulin kappa-Chains
  • Lymphoma, B-Cell / immunology*
  • Lymphoma, B-Cell / therapy
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Multiple Myeloma / immunology*
  • Multiple Myeloma / therapy
  • Peptide Fragments / immunology*
  • Recombinant Fusion Proteins / immunology
  • Splenic Neoplasms / therapy*
  • Tetanus Toxin / immunology*
  • Vaccines, DNA*

Substances

  • Cancer Vaccines
  • Immunoglobulin Fragments
  • Immunoglobulin M
  • Immunoglobulin Variable Region
  • Immunoglobulin kappa-Chains
  • Peptide Fragments
  • Recombinant Fusion Proteins
  • Tetanus Toxin
  • Vaccines, DNA
  • tetanus toxin fragment C