Water-soluble conjugate of double-stranded DNA and Poly(N-isopropylacrylamide) for one-pot affinity precipitation separation of DNA-binding proteins

D Umeno; M Kawasaki; M Maeda

doi:10.1021/bc980019f

Water-soluble conjugate of double-stranded DNA and Poly(N-isopropylacrylamide) for one-pot affinity precipitation separation of DNA-binding proteins

Bioconjug Chem. 1998 Nov-Dec;9(6):719-24. doi: 10.1021/bc980019f.

Authors

D Umeno¹, M Kawasaki, M Maeda

Affiliation

¹ Department of Materials Physics and Chemistry, Graduate School of Engineering, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.

PMID: 9815165
DOI: 10.1021/bc980019f

Abstract

Poly(N-isopropylacrylamide) having a terminal psoralen group was synthesized and covalently bound to plasmid pBR 322 DNA through a photochemical reaction. The resulting conjugate exhibited temperature-responsive precipitation due to the aggregation of poly(N-isopropylacrylamide) chains when heated above 31 degreesC. This system was used for the one-pot separation of restriction endonuclease EcoRI.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acrylic Resins / chemistry*
Chromatography, Affinity
DNA / chemistry*
DNA-Binding Proteins / isolation & purification*
Deoxyribonuclease EcoRI / isolation & purification
Electrophoresis, Polyacrylamide Gel
Furocoumarins / chemistry
Indicators and Reagents
Photochemistry
Solubility

Substances

Acrylic Resins
DNA-Binding Proteins
Furocoumarins
Indicators and Reagents
poly-N-isopropylacrylamide
DNA
Deoxyribonuclease EcoRI