Abstract
IL-4 is produced promptly in response to certain infections and plays a key role in the Th1/Th2 T cell dichotomy; however, the cellular source remains a matter of debate. Here we describe the induction of IL-4 in bone marrow cells of normal and RAG-/- mice by both Mycobacterium tuberculosis and its major cell wall glycolipid, lipoarabinomannan. Characterization of the cell type responsible indicated that it was distinct from the NK1+ or CD4+ T cell previously ascribed the function of rapid IL-4 secretion. Cell-sorting experiments identified CD19+/B220+ precursor cells, presumably pre-B cells that produced IL-4 constitutively and whose frequency was rapidly and markedly up-regulated by lipoarabinomannan. Thus, pathogenic mycobacteria and their glycolipids may influence hemopoiesis by rapidly inducing IL-4 secretion in the bone marrow.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens / analysis
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Antigens, Bacterial / metabolism
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Antigens, Bacterial / pharmacology*
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Antigens, Surface
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Arabinose / immunology
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B-Lymphocyte Subsets / immunology
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B-Lymphocyte Subsets / metabolism*
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Bone Marrow Cells / immunology
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Bone Marrow Cells / metabolism*
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Cell Wall / immunology
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Female
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Hematopoietic Stem Cells / immunology
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Hematopoietic Stem Cells / metabolism*
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Immunophenotyping
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Interleukin-4 / biosynthesis*
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Lectins, C-Type
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Lipopolysaccharides / metabolism
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Lipopolysaccharides / pharmacology*
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Mannose / immunology
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Knockout
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Mice, SCID
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Mycobacterium bovis / immunology
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Mycobacterium tuberculosis / immunology
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NK Cell Lectin-Like Receptor Subfamily B
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Proteins / analysis
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T-Lymphocyte Subsets / immunology
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T-Lymphocyte Subsets / metabolism
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Tuberculosis / immunology
Substances
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Antigens
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Antigens, Bacterial
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Antigens, Surface
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Lectins, C-Type
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Lipopolysaccharides
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NK Cell Lectin-Like Receptor Subfamily B
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Proteins
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lipoarabinomannan
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Interleukin-4
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Arabinose
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Mannose