Abstract
By using the yeast two-hybrid system, we identified the ribosomal protein L6/TAXREB107 as an intracellular partner for FGF-2. L6/TAXREB107 also mediates the DNA binding of the HTLV-1 transactivator Tax. In vitro binding experiments indicated that both the high-molecular-weight forms (HMW) and the 18-kDa form of FGF-2 bind to L6/TAXREB107. Deletion analysis suggested that L6/TAXREB107 has two binding sites for HMW FGF-2 and one binding site for 18-kDa FGF-2, implying that the unique N-terminal extension of the HMW FGF-2 is one of the binding domains for L6/TAXREB107. Transfection assays showed that high expression of either HMW or 18 kDa FGF-2 stimulates Tax-mediated transactivation in NIH 3T3 cells. This result suggests a possible role of FGF-2 in Tax-mediated HTLV-1 transformation as well as FGF-2 binding to ribosomes and/or their precursors.
Copyright 1998 Academic Press.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Base Sequence
-
Binding Sites / genetics
-
DNA Primers / genetics
-
DNA-Binding Proteins / chemistry
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / metabolism*
-
Fibroblast Growth Factor 2 / chemistry
-
Fibroblast Growth Factor 2 / genetics
-
Fibroblast Growth Factor 2 / metabolism*
-
Gene Products, tax / chemistry
-
Gene Products, tax / genetics
-
Gene Products, tax / metabolism*
-
In Vitro Techniques
-
Mice
-
Molecular Weight
-
Polymerase Chain Reaction
-
Recombinant Proteins / chemistry
-
Recombinant Proteins / genetics
-
Recombinant Proteins / metabolism
-
Ribosomal Proteins / chemistry
-
Ribosomal Proteins / genetics
-
Ribosomal Proteins / metabolism*
-
Saccharomyces cerevisiae / genetics
-
Saccharomyces cerevisiae / metabolism
-
Sequence Deletion
-
Transcriptional Activation
-
Transfection
Substances
-
DNA Primers
-
DNA-Binding Proteins
-
Gene Products, tax
-
Recombinant Proteins
-
Ribosomal Proteins
-
ribosomal protein L6
-
tax-responsive-element-binding protein 107, mouse
-
Fibroblast Growth Factor 2