Alzheimer-specific epitope of AT100 in transfected cell lines with tau: toward an efficient cell model of tau abnormal phosphorylation

Neurosci Lett. 1998 Oct 9;255(1):13-6. doi: 10.1016/s0304-3940(98)00693-4.

Abstract

Intraneuronal aggregation of specific hyperphosphorylated tau isoforms in subsets of neurons may explain many neurodegenerative processes. Only some antibodies including AP422 and AT100 are specific to the abnormal phosphorylation of tau proteins in these processes. AT100-immunoreactivity was never observed in cell models with the exception of Sf9 cells. In the present study, we developed a way to induce AT100-immunoreactivity in different cell types including COS and SY5Y cells after tau cDNA transfection and treatment by okadaic acid. This represents a useful model to study abnormal tau phosphorylation in situ.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / immunology*
  • Animals
  • Antibodies, Monoclonal / immunology*
  • COS Cells / drug effects
  • COS Cells / metabolism
  • Cell Line, Transformed
  • DNA, Complementary / genetics
  • Electrophoresis, Polyacrylamide Gel
  • Epitopes / immunology*
  • Epitopes / metabolism*
  • Okadaic Acid / pharmacology
  • Phosphorylation
  • Transfection / genetics
  • Tumor Cells, Cultured / drug effects
  • tau Proteins / genetics
  • tau Proteins / metabolism*

Substances

  • Antibodies, Monoclonal
  • DNA, Complementary
  • Epitopes
  • tau Proteins
  • Okadaic Acid