Abstract
Clim-2 (NLI, Lbd1) is one of two related mouse proteins that interact with Lim-domain homeoproteins. In the mouse, embryonic expression of Clim-2 is particularly pronounced in facial ectomesenchyme and limb bud mesenchyme in association with Lim genes, Lhx-6 and Lmx-1 respectively. We show that in common with both these Lim genes, Clim-2 expression is regulated by signals from overlying epithelium. In both the developing face and the limb buds we identify Fgf-8 as the likely candidate signalling molecule that regulates Clim-2 expression. We show that in the mandibular arch, as in the limb, Fgf-8 functions in combination with CD44, a cell surface binding protein, and that blocking CD44 binding results in inhibition of Fgf8-induced expression of Clim-2 and Lhx-6. Regulation of gene expression by Fgf8 in association with CD44 is thus conserved between limb and mandibular arch development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bone Morphogenetic Proteins / genetics
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DNA-Binding Proteins*
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Embryonic and Fetal Development*
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Extremities / growth & development*
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Fibroblast Growth Factor 8
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Fibroblast Growth Factor 9
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Fibroblast Growth Factors / genetics*
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Gene Expression Regulation, Developmental / genetics*
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Growth Substances / genetics
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Homeodomain Proteins / genetics
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Hyaluronan Receptors / genetics
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Hyaluronic Acid / pharmacology
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In Situ Hybridization
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LIM Domain Proteins
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Mandible / growth & development*
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Mesoderm / metabolism*
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Mice
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Mice, Inbred Strains
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Transcription Factors / genetics
Substances
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Bone Morphogenetic Proteins
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DNA-Binding Proteins
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Fgf8 protein, mouse
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Fgf9 protein, mouse
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Fibroblast Growth Factor 9
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Growth Substances
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Homeodomain Proteins
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Hyaluronan Receptors
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LIM Domain Proteins
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Ldb1 protein, mouse
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Transcription Factors
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Fibroblast Growth Factor 8
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Fibroblast Growth Factors
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Hyaluronic Acid