Abstract
Cell cycle progression is dependent on the sequential activity of cyclin-dependent kinases (CDKs). For full activity, CDKs require an activating phosphorylation of a conserved residue (corresponding to Thr160 in human CDK2) carried out by the CDK-activating kinase (CAK). Two distinct CAK kinases have been described: in budding yeast Saccharomyces cerevisiae, the Cak1/Civ1 kinase is responsible for CAK activity. In several other species including human, Xenopus, Drosophila and fission yeast Schizosaccharomyces pombe, CAK has been identified as a complex homologous to CDK7-cyclin H (Mcs6-Mcs2 in fission yeast). Here we identify the fission yeast Csk1 kinase as an in vivo activating kinase of the Mcs6-Mcs2 CAK defining Csk1 as a CAK-activating kinase (CAKAK).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Cell Cycle
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Conserved Sequence
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Cyclin H
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Cyclin-Dependent Kinase-Activating Kinase
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Cyclin-Dependent Kinases / metabolism*
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Cyclins / genetics
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Cyclins / metabolism
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Drosophila Proteins
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Enzyme Activation
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Gene Dosage
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Genes, Suppressor
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Phosphorylation
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Protein Kinases / metabolism*
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Schizosaccharomyces / enzymology*
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Schizosaccharomyces pombe Proteins*
Substances
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CCNH protein, human
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CycH protein, Drosophila
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Cyclin H
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Cyclins
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Drosophila Proteins
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Schizosaccharomyces pombe Proteins
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Protein Kinases
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Csk1 protein, S pombe
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Protein Serine-Threonine Kinases
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Cyclin-Dependent Kinases
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Cyclin-Dependent Kinase-Activating Kinase