Abstract
The expression pattern of the fibroblast growth factor receptor Fgf-R1, R2 and R3 genes was studied in the chicken spinal cord using in situ hybridization (ISH). Unlike Fgf-R1 which is widely expressed in motoneurons, Fgf-R3 is expressed in a subset of motoneurons in the medial subdivision of the median motor column (MMCm) that also express Islet-1 and Lim-3. The motoneuron identity of the labelled cells was confirmed by double ISH and by single cell RT-PCR. Interestingly, E3.5 spinal cord motoneurons do not express Fgf-R3, suggesting the expression of Fgf-R3 in motoneurons begins with axonal growth.
Copyright 1998 Elsevier Science Ireland Ltd. All Rights Reserved
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Chick Embryo
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Gene Expression Regulation, Developmental*
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Homeodomain Proteins / biosynthesis
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Homeodomain Proteins / genetics
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In Situ Hybridization
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LIM-Homeodomain Proteins
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Molecular Sequence Data
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Motor Neurons / classification
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Motor Neurons / metabolism*
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Nerve Tissue Proteins / biosynthesis*
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Nerve Tissue Proteins / genetics
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Protein Isoforms / biosynthesis*
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Protein Isoforms / genetics
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Protein-Tyrosine Kinases*
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Receptor Protein-Tyrosine Kinases / biosynthesis
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Receptor Protein-Tyrosine Kinases / genetics
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Receptor, Fibroblast Growth Factor, Type 1
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Receptor, Fibroblast Growth Factor, Type 3
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Receptors, Fibroblast Growth Factor / biosynthesis*
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Receptors, Fibroblast Growth Factor / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Spinal Cord / cytology
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Spinal Cord / embryology*
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Transcription Factors
Substances
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Homeodomain Proteins
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LIM-Homeodomain Proteins
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Nerve Tissue Proteins
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Protein Isoforms
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Receptors, Fibroblast Growth Factor
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Transcription Factors
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insulin gene enhancer binding protein Isl-1
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Protein-Tyrosine Kinases
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Receptor Protein-Tyrosine Kinases
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Receptor, Fibroblast Growth Factor, Type 1
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Receptor, Fibroblast Growth Factor, Type 3