Globin chain synthesis analysis by high performance liquid chromatography in the screening of thalassemia syndromes

Hemoglobin. 1998 Sep-Nov;22(5-6):501-8. doi: 10.3109/03630269809071547.

Abstract

We applied reversed phase high performance liquid chromatography for globin chain synthesis analysis in screening for beta-thalassemia. The alpha/non-alpha-globin chain synthesis ratios have been determined in alpha-, beta-, and deltabeta-thalassemia carriers using the classical carboxymethyl cellulose chromatography as the reference method. Reversed phase high performance liquid chromatography is fast, accurate, and reproducible, and may be a suitable alternative for the traditional carboxymethyl cellulose chromatography.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carboxymethylcellulose Sodium
  • Chromatography / methods
  • Chromatography, High Pressure Liquid*
  • Erythrocytes
  • Evaluation Studies as Topic
  • Globins / biosynthesis*
  • Globins / chemistry
  • Humans
  • beta-Thalassemia / blood
  • beta-Thalassemia / diagnosis*
  • beta-Thalassemia / immunology

Substances

  • Globins
  • Carboxymethylcellulose Sodium