Abstract
Synthesis of mannopine in plant tissues infected with Agrobacterium tumefaciens is controlled by a divergent promoter (pmas2' and pmas1') that in 479 bp contains all the cis-acting elements necessary to direct tissue-specific and wound-inducible expression. In this report, using transgenic tobacco plants harboring a pmas1'-beta-glucuronidase (GUS) gene fusion, we investigated the developmental expression pattern directed by pmas1' in the early stages of development and the responses of pmas1' to different chemical inducers. It was found that this promoter can respond to auxins, cytokinins, methyl jasmonate (MJ), salicylic acid (SA) and its analogue 2,6-dichloroisonicotinic acid (iNA). Treatment with chemical inducers also showed that the effects of iNA are organ-dependent, that wound-induction is a complex response mediated by at least two different chemical signals, and that MJ stimulates changes in the tissue-specific and developmental expression pattern directed by the ptmas1' promoter. Using chimeric promoters we demonstrate that an ocs-like element (ocs+1) directs MJ responses in an orientation-dependent manner and that sequences around the ocs+1 are important to maintain the inducible and developmental properties of this cis-regulatory element.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetates / pharmacology
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Agrobacterium tumefaciens / genetics
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Amino Acid Oxidoreductases / genetics*
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Cyclopentanes / pharmacology
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Cytokinins / pharmacology
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DNA / genetics
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Gene Expression Regulation, Developmental / drug effects
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Gene Expression Regulation, Plant / drug effects
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Genes, Plant / genetics*
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Glucuronidase / drug effects
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Glucuronidase / genetics
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Glucuronidase / metabolism
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Histocytochemistry
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Hydro-Lyases / genetics*
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Indoleacetic Acids / pharmacology
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Isonicotinic Acids / pharmacology
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Nicotiana / genetics
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Oxylipins
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Plant Leaves / drug effects
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Plant Leaves / genetics
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Plant Roots / drug effects
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Plant Roots / genetics
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Plants, Genetically Modified
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Plants, Toxic
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Plasmids / genetics
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Promoter Regions, Genetic / drug effects
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Promoter Regions, Genetic / genetics
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Recombinant Fusion Proteins / drug effects
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Regulatory Sequences, Nucleic Acid*
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Salicylic Acid / pharmacology
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Sequence Deletion
Substances
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Acetates
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Cyclopentanes
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Cytokinins
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Indoleacetic Acids
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Isonicotinic Acids
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Oxylipins
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Recombinant Fusion Proteins
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2,6-dichloroisonicotinic acid
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DNA
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methyl jasmonate
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Amino Acid Oxidoreductases
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D-octopine dehydrogenase
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Glucuronidase
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Hydro-Lyases
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mannopine synthase
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Salicylic Acid