Ischemic preconditioning mediated by calcitonin gene-related peptide in isolated rat hearts

Zhongguo Yao Li Xue Bao. 1996 Sep;17(5):445-8.

Abstract

Aim: To study the mediating effect of calcitonin gene-related peptide (CGRP) in ischemic preconditioning in the isolated perfused rat heart.

Methods: Isolated rat hearts were subjected to 3 cycles of a 5-min ischemia and a 5-min reperfusion before a 30-min global ischemia followed by a 30-min reperfusion.

Results: Ischemic preconditioning caused an improvement of heart functions, reduced the incidence of ventricular arrhythmias and decreased the release of creatine kinase (CK) during reperfusion (CK activities = 0.30 +/- 0.07, 2.03 +/- 0.49, and 0.92 +/- 0.40 U.min-1/g wet wt for control, ischemia-reperfusion, and preconditioning, respectively). However, pretreatment with CGRP8-37 (0.1 mumol.L-1) abolished the improvement of cardiac contractivity, the reduction of the incidence of arrhythmias, and the inhibition of CK release by preconditioning (CK activities = 0.9 +/- 0.4 vs 2.55 +/- 0.32 U.min-1/g wet wt, P < 0.01).

Conclusion: CGRP is an endogenous myocardial protective substance that played an important role in mediation of ischemic preconditioning.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arrhythmias, Cardiac / prevention & control
  • Calcitonin Gene-Related Peptide / pharmacology*
  • Coronary Circulation / drug effects
  • Creatine Kinase / metabolism*
  • Heart Rate / drug effects
  • In Vitro Techniques
  • Ischemic Preconditioning, Myocardial*
  • Male
  • Peptide Fragments / pharmacology
  • Rats
  • Rats, Wistar

Substances

  • Peptide Fragments
  • calcitonin gene-related peptide (8-37)
  • Creatine Kinase
  • Calcitonin Gene-Related Peptide