Abstract
We analyzed 92 Cryptosporidium parvum isolates from humans and animals by a polymerase chain reaction/restriction fragment length polymorphism method based on the thrombospondin-related anonymous protein 2 gene sequence. Used as a molecular marker, this method can differentiate between the two genotypes of C. parvum and elucidate the transmission of infection to humans.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Cattle
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Cattle Diseases / microbiology*
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Cryptosporidiosis / epidemiology
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Cryptosporidiosis / parasitology*
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Cryptosporidiosis / veterinary*
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Cryptosporidium parvum / classification*
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Cryptosporidium parvum / genetics
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Cryptosporidium parvum / isolation & purification
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Disease Outbreaks*
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Genotype
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Humans
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length
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Protozoan Proteins / genetics*
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Sequence Analysis, DNA
Substances
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Protozoan Proteins
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thrombospondin-related adhesive protein, protozoan