Perianal Paget's disease: distinguishing primary and secondary lesions using immunohistochemical studies including gross cystic disease fluid protein-15 and cytokeratin 20 expression

Arch Pathol Lab Med. 1998 Dec;122(12):1077-81.

Abstract

Background: Extramammary Paget's disease most commonly occurs on the female external genitalia and rarely occurs in the perianal region and male external genitalia. We present the clinical and pathologic features of 5 cases of perianal Paget's disease and review the literature.

Methods: Clinical and pathologic data were recorded for 5 cases of perianal Paget's disease. Cases were studied retrospectively with special stains, including periodic acid-Schiff, mucicarmine, Alcian blue, carcinoembryonic antigen, S100 protein, pan-keratin, gross cystic disease fluid protein-15 (GCDFP-15), lysozyme, CD15 (Leu-M1), cytokeratin 7 (CK7), and cytokeratin 20 (CK20).

Results: Three (60%) of 5 patients had concurrent rectal adenocarcinomas. All cases reacted positively for pankeratin, although the intensity and distribution of staining varied. Both cases not associated with an underlying carcinoma showed strong GCDFP-15 and CK7 expression and an absence of CK20 expression. The 3 cases associated with an underlying malignancy demonstrated CK7 and CK20 expression and an absence of GCDFP-15 expression. All cases were negative for lysozyme and CD15 (Leu-M1).

Conclusions: The 5 cases reported herein demonstrate that perianal Paget's disease is a heterogeneous entity. The high frequency of associated underlying malignancies and resultant poor clinical outcomes highlight the importance of an aggressive search for a second malignancy. In some cases, perianal Paget's disease merely represents a cutaneous manifestation of an underlying rectal adenocarcinoma and demonstrates a CK7+/CK20+/GCDFP-15-/lysozyme-/Leu-M1- immunophenotype and signet ring Paget's cells. Other cases represent primary adenocarcinomas of the skin, which are associated with a CK7+/CK20-/GCDFP-15+/lysozyme /Leu-M1- immunophenotype and an excellent prognosis if adequately resected. Immunohistochemical studies, particularly CK20 and GCDFP-15, are useful adjuncts in distinguishing primary and secondary perianal Paget's disease.

Publication types

  • Case Reports
  • Review

MeSH terms

  • Aged
  • Anus Neoplasms / metabolism
  • Anus Neoplasms / pathology*
  • Apolipoproteins D
  • Apolipoproteins*
  • Biomarkers, Tumor / metabolism
  • Carcinoembryonic Antigen / metabolism
  • Carrier Proteins / metabolism*
  • Fatal Outcome
  • Female
  • Glycoproteins*
  • Humans
  • Immunohistochemistry
  • Intermediate Filament Proteins / metabolism
  • Keratin-20
  • Keratin-7
  • Keratins / metabolism
  • Lewis X Antigen / metabolism
  • Male
  • Membrane Transport Proteins*
  • Middle Aged
  • Muramidase / metabolism
  • Paget Disease, Extramammary / metabolism
  • Paget Disease, Extramammary / pathology*
  • Retrospective Studies

Substances

  • APOD protein, human
  • Apolipoproteins
  • Apolipoproteins D
  • Biomarkers, Tumor
  • Carcinoembryonic Antigen
  • Carrier Proteins
  • Glycoproteins
  • Intermediate Filament Proteins
  • KRT20 protein, human
  • KRT7 protein, human
  • Keratin-20
  • Keratin-7
  • Lewis X Antigen
  • Membrane Transport Proteins
  • PIP protein, human
  • Keratins
  • Muramidase