Modulation of interleukin-8 activity by gingipains from Porphyromonas gingivalis: implications for pathogenicity of periodontal disease

FEBS Lett. 1998 Dec 4;440(3):282-6. doi: 10.1016/s0014-5793(98)01461-6.

Abstract

Gingipains are the major cysteine proteinases synthesized by Porphyromonas gingivalis which, in soluble form, are able to initially convert IL-8 (77 amino acid residues) to a more potent species truncated at the amino terminus, followed by slow degradation and destruction of chemokine biological activity. In contrast, the same enzymes when associated with bacterial outer-membrane blebs (vesicles), instantly degrade this chemokine. This division of enhancing and inactivating activity between soluble and membrane-bound gingipains can cause the compartmentalization of pro- and anti-inflammatory reactions to distal and proximal positions from bacterial plaque, respectively, which may explain why, despite the massive neutrophil accumulation at periodontitis sites, there is no elimination of infection.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adhesins, Bacterial
  • Cysteine Endopeptidases / physiology*
  • Gingipain Cysteine Endopeptidases
  • Hemagglutinins / physiology*
  • Humans
  • In Vitro Techniques
  • Interleukin-8 / metabolism*
  • Neutrophil Activation
  • Neutrophils / immunology*
  • Peptide Hydrolases / metabolism
  • Periodontal Diseases / immunology*
  • Periodontal Diseases / microbiology
  • Porphyromonas gingivalis / enzymology
  • Porphyromonas gingivalis / immunology*
  • Porphyromonas gingivalis / pathogenicity
  • Recombinant Proteins / metabolism

Substances

  • Adhesins, Bacterial
  • Gingipain Cysteine Endopeptidases
  • Hemagglutinins
  • Interleukin-8
  • Recombinant Proteins
  • Peptide Hydrolases
  • Cysteine Endopeptidases