A synthetic acceptor substrate for Trypanosoma brucei UDP-Gal: GPI anchor side-chain alpha-galactosyltransferases

Bioorg Med Chem Lett. 1998 Aug 4;8(15):2051-4. doi: 10.1016/s0960-894x(98)00359-x.

Abstract

A synthetic analogue of a trisaccharide fragment of the Trypanosoma brucei Variant Surface Glycoprotein (VSG) GlycosylPhosphatidyInositiol (GPI) anchor, Gal-alpha-1,3(Man-alpha-1,6)-Man-alpha-O-octyl (1), serves as a substrate for two T. brucei alpha-galactosyltransferases. The principle tetrasaccharide product derived from (1) contains a Gal-alpha-1,2-Gal linkage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biotransformation
  • Carbohydrate Sequence
  • Galactosyltransferases / metabolism*
  • Glycosylphosphatidylinositols / metabolism*
  • Molecular Sequence Data
  • Substrate Specificity
  • Trypanosoma brucei brucei / enzymology*
  • Uridine Diphosphate Galactose / metabolism*
  • Variant Surface Glycoproteins, Trypanosoma / metabolism*

Substances

  • Glycosylphosphatidylinositols
  • Variant Surface Glycoproteins, Trypanosoma
  • Uridine Diphosphate Galactose
  • Galactosyltransferases