Synthesis and hybridization property of an oligonucleotide analog containing a 1',3'-di-O-methylene-alpha-D-fructose backbone

R Zou; M D Matteucci

doi:10.1016/S0960-894X(98)00566-6

Synthesis and hybridization property of an oligonucleotide analog containing a 1',3'-di-O-methylene-alpha-D-fructose backbone

Bioorg Med Chem Lett. 1998 Nov 3;8(21):3049-52. doi: 10.1016/S0960-894X(98)00566-6.

Authors

R Zou¹, M D Matteucci

Affiliation

¹ Gilead Sciences, Inc., Foster City, CA 94404, USA.

PMID: 9873674
DOI: 10.1016/S0960-894X(98)00566-6

Abstract

Hydrogen phosphonate monomers of T (thymine) and C(m) (5-methylcytosine) bearing a 1',3'-di-O-methylene-alpha-D-fructose sugar moiety were synthesized and incorporated into an oligonucleotide. Hybridization studies by thermal denaturation experiment indicated that this oligonucleotide did not form a duplex with the complementary RNA target.

MeSH terms

Fructose
Nucleic Acid Hybridization*
Oligonucleotides, Antisense / chemical synthesis*
Oligonucleotides, Antisense / chemistry

Substances

Oligonucleotides, Antisense
Fructose