Targeted mutagenesis by duplication insertion in the radioresistant bacterium Deinococcus radiodurans: radiation sensitivities of catalase (katA) and superoxide dismutase (sodA) mutants

L M Markillie; S M Varnum; P Hradecky; K K Wong

doi:10.1128/JB.181.2.666-669.1999

Targeted mutagenesis by duplication insertion in the radioresistant bacterium Deinococcus radiodurans: radiation sensitivities of catalase (katA) and superoxide dismutase (sodA) mutants

J Bacteriol. 1999 Jan;181(2):666-9. doi: 10.1128/JB.181.2.666-669.1999.

Authors

L M Markillie¹, S M Varnum, P Hradecky, K K Wong

Affiliation

¹ Pacific Northwest National Laboratory, Molecular Biosciences, Richland, Washington 99352, USA.

Abstract

Deinococcus radiodurans R1 is extremely resistant to both oxidative stress and ionizing radiation. A simple and general targeted mutagenesis method was developed to generate catalase (katA) and superoxide dismutase (sodA) mutants. Both mutants were shown to be more sensitive to ionizing radiation than the wild type.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Catalase / genetics*
Cobalt Radioisotopes
Dose-Response Relationship, Radiation
Escherichia coli / genetics
Gamma Rays*
Micrococcus / enzymology
Micrococcus / genetics*
Micrococcus / radiation effects*
Mutagenesis, Insertional*
Oxidative Stress
Radiation Tolerance
Superoxide Dismutase / genetics*

Substances

Cobalt Radioisotopes
Catalase
Superoxide Dismutase