Abstract
PCAF histone acetylase is found in a complex with more than 20 associated polypeptides. Here we report cloning and characterization of the 400 kDa PCAF-associated factor referred to as PAF400. PAF400 is almost identical to TRRAP, which binds to c-Myc and E2F, and has significant sequence similarities to the ATM superfamily including FRAP, ATM, ATR, and the catalytic subunit of DNA-PK. Remarkably, PAF400 and FRAP share sequence similarity in broad regions that cover 80% of the entire PAF400 sequence. However, unlike the other members of the ATM superfamily, PAF400 is not a protein kinase as judged from the lack of kinase motif and autophosphorylation activity. We discuss the possibility that PAF400 may play a role in signaling of DNA damage to p53 by stimulation of p53 acetylation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyltransferases / genetics*
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Acetyltransferases / metabolism
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Amino Acid Sequence
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Ataxia Telangiectasia Mutated Proteins
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Base Sequence
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Cell Cycle Proteins
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Cloning, Molecular
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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DNA-Binding Proteins
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HeLa Cells
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Histone Acetyltransferases
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Humans
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Molecular Sequence Data
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Phosphorylation
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Protein Serine-Threonine Kinases*
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Proteins / genetics*
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Proteins / metabolism
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Tumor Suppressor Proteins
Substances
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Cell Cycle Proteins
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DNA, Complementary
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DNA-Binding Proteins
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Proteins
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Saccharomyces cerevisiae Proteins
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Tumor Suppressor Proteins
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Acetyltransferases
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Histone Acetyltransferases
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ATM protein, human
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Ataxia Telangiectasia Mutated Proteins
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Protein Serine-Threonine Kinases