Abstract
Human interleukin-17 (hIL-17) gene without the signal sequence was isolated from activated peripheral blood lymphocytes by RT-PCR, then highly expressed in the yeast Pichia pastoris in the form of the glycosylated monomer. The monomer of rhIL-17 stimulated mouse fibroblast 3T3 cells to secrete IL-6 and was specifically bound to its receptors on 3T3 cells.
MeSH terms
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3T3 Cells
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Animals
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Cloning, Molecular
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DNA Primers
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Humans
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Interleukin-17 / biosynthesis*
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Interleukin-17 / genetics
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Interleukin-17 / pharmacology
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Interleukin-6 / biosynthesis
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Kinetics
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Lymphocyte Activation
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Lymphocytes / immunology
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Mice
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Pichia
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Polymerase Chain Reaction
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Receptors, Interleukin / metabolism
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Receptors, Interleukin-17
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / metabolism
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Recombinant Proteins / pharmacology
Substances
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DNA Primers
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IL17RA protein, human
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Il17ra protein, mouse
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Interleukin-17
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Interleukin-6
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Receptors, Interleukin
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Receptors, Interleukin-17
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Recombinant Proteins