Fcgamma receptor-mediated induction of dendritic cell maturation and major histocompatibility complex class I-restricted antigen presentation after immune complex internalization

A Regnault; D Lankar; V Lacabanne; A Rodriguez; C Théry; M Rescigno; T Saito; S Verbeek; C Bonnerot; P Ricciardi-Castagnoli; S Amigorena

doi:10.1084/jem.189.2.371

Fcgamma receptor-mediated induction of dendritic cell maturation and major histocompatibility complex class I-restricted antigen presentation after immune complex internalization

J Exp Med. 1999 Jan 18;189(2):371-80. doi: 10.1084/jem.189.2.371.

Authors

A Regnault¹, D Lankar, V Lacabanne, A Rodriguez, C Théry, M Rescigno, T Saito, S Verbeek, C Bonnerot, P Ricciardi-Castagnoli, S Amigorena

Affiliation

¹ Institut National de la Santé et de la Recherche Médicale CJF 95-01, Institut Curie, Section Recherche, 75005 Paris, France.

Abstract

Dendritic cells (DCs) express several receptors for the Fc portion of immunoglobulin (Ig)G (FcgammaR), which mediate internalization of antigen-IgG complexes (immune complexes, ICs) and promote efficient major histocompatibility complex (MHC) class II-restricted antigen presentation. We now show that FcgammaRs have two additional specific attributes in murine DCs: the induction of DC maturation and the promotion of efficient MHC class I-restricted presentation of peptides from exogenous, IgG-complexed antigens. Both FcgammaR functions require the FcgammaR-associated gamma chain. FcgammaR-mediated MHC class I-restricted antigen presentation is extremely sensitive and specific to immature DCs. It requires proteasomal degradation and is dependent on functional peptide transporter associated with antigen processing, TAP1-TAP2. By promoting DC maturation and presentation on both MHC class I and II molecules, ICs should efficiently sensitize DCs for priming of both CD4(+) helper and CD8(+) cytotoxic T lymphocytes in vivo.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ATP Binding Cassette Transporter, Subfamily B, Member 2
ATP Binding Cassette Transporter, Subfamily B, Member 3
ATP-Binding Cassette Transporters / immunology
Animals
Antigen Presentation / immunology*
Antigen-Antibody Complex / immunology*
Antigens, CD / immunology
Bone Marrow / immunology
Cysteine Endopeptidases / metabolism
Dendritic Cells / immunology*
Fluorescent Antibody Technique
Histocompatibility Antigens Class I / immunology*
Immunoglobulin G / immunology
Lipopolysaccharides / pharmacology
Mice
Mice, Knockout
Multienzyme Complexes / metabolism
Ovalbumin / immunology
Proteasome Endopeptidase Complex
Receptors, IgG / immunology*

Substances

ATP Binding Cassette Transporter, Subfamily B, Member 2
ATP Binding Cassette Transporter, Subfamily B, Member 3
ATP-Binding Cassette Transporters
Antigen-Antibody Complex
Antigens, CD
Histocompatibility Antigens Class I
Immunoglobulin G
Lipopolysaccharides
Multienzyme Complexes
Receptors, IgG
TAP1 protein, human
Tap1 protein, mouse
Tap2 protein, mouse
TAP2 protein, human
Ovalbumin
Cysteine Endopeptidases
Proteasome Endopeptidase Complex