Managing and manipulating carbocations in biology: terpenoid cyclase structure and mechanism

Curr Opin Struct Biol. 1998 Dec;8(6):695-703. doi: 10.1016/s0959-440x(98)80088-2.

Abstract

Terpenoid cyclases catalyze remarkably complex cyclization cascades that are initiated by the formation of a highly reactive carbocation in a polyisoprene substrate. Recent crystal structures of terpenoid cyclases show how these enzymes provide a template for binding and stabilizing the flexible substrate in the precise orientation required for catalysis, trigger carbocation formation, chaperone the conformations of the reactive carbocation intermediates through a unique cyclization sequence, and sequester and stabilize carbocations from premature quenching. Notably, terpenoid cyclases and catalytic antibodies have converged to similar chemical and structural strategies for managing highly reactive carbocations in polyisoprene cyclization cascades.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Alkyl and Aryl Transferases / chemistry*
  • Alkyl and Aryl Transferases / metabolism
  • Antibodies, Catalytic / chemistry
  • Antibodies, Catalytic / metabolism
  • Cations
  • Models, Molecular
  • Protein Conformation

Substances

  • Antibodies, Catalytic
  • Cations
  • Alkyl and Aryl Transferases
  • terpene synthase