High-pressure destruction kinetics of Listeria monocytogenes on pork

J Food Prot. 1999 Jan;62(1):40-5. doi: 10.4315/0362-028x-62.1.40.

Abstract

Packaged fresh pork chops (30-g samples) containing an indigenous bacterial population of approximately 10(7) CFU/g were inoculated with 10(7) CFU of Listeria monocytogenes Scott A per g, heat sealed, and subjected to high-pressure processing at 200 to 400 MPa for up to 90 min. Total counts and the number of surviving L. monocytogenes cells were determined by a spread plate technique on tryptic soy agar and modified Oxford medium, respectively. The pressure destruction was characterized by a dual-behavior, consisting of a step change in the number of survivors (Pk0) with the application of a pressure pulse and a first-order rate drop in the number of survivors during the pressure hold period. Higher pressures resulted in higher rates of microbial inactivation, as indicated by their associated lower D values (and higher k values). The pressure sensitivities of the kinetic parameters were evaluated on the basis of Arrhenius and pressure death time (PDT)-type models. The results suggested that L. monocytogenes was more resistant to pressure inactivation than the indigenous microflora (the volume change of activation, deltaV* [Arrhenius model]), and Zp values (PDT model) were -4.17 x 10(-5) m3 mole(-1) and 134 MPa for indigenous microflora and -3.43 x 10(-5) m3 mole(-1) and 163 MPa for L. monocytogenes respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Colony Count, Microbial
  • Food Handling*
  • Food Microbiology
  • Humans
  • Hydrostatic Pressure*
  • Kinetics
  • Listeria monocytogenes / growth & development*
  • Meat / microbiology*
  • Meat-Packing Industry
  • Swine