Abstract
ClC-2 belongs to a large family of chloride channels and its expression in certain cell types is associated with the appearance of swelling-activated chloride (Cl-) currents. In the present report, we examined the hypothesis that ClC-2 plays a role in regulatory volume decrease by expressing ClC-2 in Sf9 cells using the baculovirus system. First, we showed that ClC-2 protein expression is associated with appearance of a Cl- conductance which is activated by hypo-osmotic shock and can be distinguished from swelling-activated chloride currents endogenous to Sf9 cells on the basis of its pharmacology and specific inhibition by an anti-ClC-2 antibody. Second, we show that the rate of regulatory volume decrease is significantly enhanced in Sf9 cells expressing ClC-2 protein. Hence, our data support the hypothesis that ClC-2 is capable of mediating regulatory volume decrease.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / pharmacology
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Animals
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Antibodies / pharmacology
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CLC-2 Chloride Channels
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Cell Line
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Cell Size / drug effects
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Chloride Channels / genetics
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Chloride Channels / immunology
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Chloride Channels / metabolism*
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Chloride Channels / physiology
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Gene Expression
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Gramicidin / pharmacology
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Immunoblotting
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In Vitro Techniques
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Light
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Meglumine / pharmacology
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / immunology
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Nerve Tissue Proteins / metabolism*
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Nerve Tissue Proteins / physiology
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Nitrobenzoates / pharmacology
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Osmotic Pressure / drug effects
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Patch-Clamp Techniques
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Perfusion
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Rats
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Spodoptera
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Transfection
Substances
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Antibodies
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CLC-2 Chloride Channels
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Chloride Channels
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Nerve Tissue Proteins
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Nitrobenzoates
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Gramicidin
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5-nitro-2-(3-phenylpropylamino)benzoic acid
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Meglumine
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4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid