Abstract
Phosphoinositide 3-kinase (PI 3-K) activity increases in HL-60 cells that are induced to granulocytic differentiation by all-trans-retinoic acid. Immunochemical and immunocytochemical analyses by confocal microscopy also reveal an increase in the amount of the enzyme, which is particularly evident at the nuclear level. Inhibition of PI 3-K activity by nanomolar concentrations of wortmannin and of its expression by transfection with an antisense fragment of p85alpha prevented the differentiative process. The data obtained indicate that PI 3-K activity plays an essential role in promoting granulocytic differentiation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / pharmacology*
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Cell Differentiation / drug effects
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Down-Regulation
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Granulocytes / cytology
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Granulocytes / drug effects*
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Granulocytes / enzymology*
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HL-60 Cells / cytology
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HL-60 Cells / drug effects*
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HL-60 Cells / enzymology*
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Humans
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Microscopy, Confocal
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Phosphatidylinositol 3-Kinases / metabolism*
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Phosphatidylinositol Phosphates / metabolism
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Subcellular Fractions / enzymology
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Tretinoin / pharmacology*
Substances
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Antineoplastic Agents
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Phosphatidylinositol Phosphates
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phosphatidylinositol 3,4,5-triphosphate
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Tretinoin
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Phosphatidylinositol 3-Kinases