Comparison of five PCR methods for detection of Helicobacter pylori DNA in gastric tissues

J Clin Microbiol. 1999 Mar;37(3):772-4. doi: 10.1128/JCM.37.3.772-774.1999.

Abstract

Five different PCR methods for the detection of Helicobacter pylori were evaluated. The results of this study indicate that of the five PCR methods examined, the ureC (glmM) gene PCR is the most sensitive and specific for the detection of H. pylori in gastric biopsy specimens.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biopsy
  • DNA, Bacterial / analysis*
  • DNA, Bacterial / genetics
  • Gastric Mucosa / microbiology
  • Gastric Mucosa / pathology
  • Helicobacter Infections / diagnosis*
  • Helicobacter Infections / pathology
  • Helicobacter pylori / isolation & purification*
  • Humans
  • Phosphoglucomutase / genetics
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Stomach / microbiology*
  • Stomach / pathology

Substances

  • DNA, Bacterial
  • phosphoglucosamine mutase
  • Phosphoglucomutase