[Comparison of the microdilution method (PASCO), the Etest and disc-diffusion in streptococcus pneumoniae antibiotic susceptibility testing]

Rev Esp Quimioter. 1998 Dec;11(4):344-8.
[Article in Spanish]

Abstract

MIC values and inhibition zone diameters for penicillin, cefotaxime, and erythromycin where determined in 75 consecutive Streptococcus pneumoniae blood isolates by microdilution (PASCO), disc-diffusion and Etest. Using the Etest, 40% and 29.3% of the isolates showed low- or high-level resistance to penicillin and erythromycin respectively, whereas 9.3% were intermediate to cefotaxime. No high-level resistance to cefotaxime was found. No errors were found with the comparison of penicillin and cefotaxime results by microdilution and Etest, with just 2 (2.7%) and 3 (4%) minor errors appearing respectively. On the other hand, when comparing penicillin susceptibility results by disc-diffusion (1 mg oxacillin disc) and Etest, 6 (8%) major errors and 18 (24%) minor errors were found.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Culture Media
  • Diffusion
  • Indicator Dilution Techniques
  • Microbial Sensitivity Tests / methods*
  • Streptococcus pneumoniae / drug effects*

Substances

  • Anti-Bacterial Agents
  • Culture Media