Kumagai I - Search Results

1

Expression of a synthetic gene encoding canine milk lysozyme in Escherichia coli and characterization of the expressed protein.

Koshiba T, Hayashi T, Miwako I, Kumagai I, Ikura T, Kawano K, Nitta K, Kuwajima K. Koshiba T, et al. Among authors: kumagai i. Protein Eng. 1999 May;12(5):429-35. doi: 10.1093/protein/12.5.429. Protein Eng. 1999. PMID: 10360984

2

Effects of amino acid substitutions in the hydrophobic core of alpha-lactalbumin on the stability of the molten globule state.

Uchiyama H, Perez-Prat EM, Watanabe K, Kumagai I, Kuwajima K. Uchiyama H, et al. Among authors: kumagai i. Protein Eng. 1995 Nov;8(11):1153-61. doi: 10.1093/protein/8.11.1153. Protein Eng. 1995. PMID: 8819981

3

Calorimetric study of mutant human lysozymes with partially introduced Ca2+ binding sites and its efficient refolding system from inclusion bodies.

Koshiba T, Tsumoto K, Masaki K, Kawano K, Nitta K, Kumagai I. Koshiba T, et al. Among authors: kumagai i. Protein Eng. 1998 Aug;11(8):683-90. doi: 10.1093/protein/11.8.683. Protein Eng. 1998. PMID: 9749921

4

Effect of the extra n-terminal methionine residue on the stability and folding of recombinant alpha-lactalbumin expressed in Escherichia coli.

Chaudhuri TK, Horii K, Yoda T, Arai M, Nagata S, Terada TP, Uchiyama H, Ikura T, Tsumoto K, Kataoka H, Matsushima M, Kuwajima K, Kumagai I. Chaudhuri TK, et al. Among authors: kumagai i. J Mol Biol. 1999 Jan 22;285(3):1179-94. doi: 10.1006/jmbi.1998.2362. J Mol Biol. 1999. PMID: 9887272

5

Folding-unfolding of goat alpha-lactalbumin studied by stopped-flow circular dichroism and molecular dynamics simulations.

Yoda T, Saito M, Arai M, Horii K, Tsumoto K, Matsushima M, Kumagai I, Kuwajima K. Yoda T, et al. Among authors: kumagai i. Proteins. 2001 Jan 1;42(1):49-65. doi: 10.1002/1097-0134(20010101)42:1<49::aid-prot60>3.0.co;2-z. Proteins. 2001. PMID: 11093260

6

Contribution of Thr29 to the thermodynamic stability of goat alpha-lactalbumin as determined by experimental and theoretical approaches.

Horii K, Saito M, Yoda T, Tsumoto K, Matsushima M, Kuwajima K, Kumagai I. Horii K, et al. Among authors: kumagai i. Proteins. 2001 Oct 1;45(1):16-29. doi: 10.1002/prot.1119. Proteins. 2001. PMID: 11536356

7

Expression of goat alpha-lactalbumin in Escherichia coli and its refolding to biologically active protein.

Kumagai I, Takeda S, Hibino T, Miura K. Kumagai I, et al. Protein Eng. 1990 Apr;3(5):449-52. doi: 10.1093/protein/3.5.449. Protein Eng. 1990. PMID: 2190211

8

Construction of a diabody (small recombinant bispecific antibody) using a refolding system.

Takemura S, Asano R, Tsumoto K, Ebara S, Sakurai N, Katayose Y, Kodama H, Yoshida H, Suzuki M, Imai K, Matsuno S, Kudo T, Kumagai I. Takemura S, et al. Among authors: kumagai i. Protein Eng. 2000 Aug;13(8):583-8. doi: 10.1093/protein/13.8.583. Protein Eng. 2000. PMID: 10964988

9

Novel selection method for engineered antibodies using the mechanism of Fv fragment stabilization in the presence of antigen.

Tsumoto K, Nishimiya Y, Kasai N, Ueda H, Nagamune T, Ogasahara K, Yutani K, Tokuhisa K, Matsushima M, Kumagai I. Tsumoto K, et al. Among authors: kumagai i. Protein Eng. 1997 Nov;10(11):1311-8. doi: 10.1093/protein/10.11.1311. Protein Eng. 1997. PMID: 9514120

10

Immobilized oxidoreductase as an additive for refolding inclusion bodies: application to antibody fragments.

Tsumoto K, Umetsu M, Yamada H, Ito T, Misawa S, Kumagai I. Tsumoto K, et al. Among authors: kumagai i. Protein Eng. 2003 Jul;16(7):535-41. doi: 10.1093/protein/gzg064. Protein Eng. 2003. PMID: 12915732

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