The effect of amino acid substitution at position 219 of Citrobacter freundii cephalosporinase on extension of its substrate spectrum.
Tsukamoto K, Ohno R, Nukaga M, Sawai T.
Tsukamoto K, et al. Among authors: sawai t.
Eur J Biochem. 1992 Aug 1;207(3):1123-7. doi: 10.1111/j.1432-1033.1992.tb17150.x.
Eur J Biochem. 1992.
PMID: 1499556
Free article.
The substitution of the glutamic acid at position 219 in the enzyme by lysine was previously shown to broaden its substrate specificity to unfavorable substrates such as oxyimino cephalosporins [Tsukamoto, K., Ohno, R. & Sawai, T. (1990) J. Bacteriol. 172, 4348- …
The substitution of the glutamic acid at position 219 in the enzyme by lysine was previously shown to broaden its substrate specificity to u …