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Converting trypsin to chymotrypsin: residue 172 is a substrate specificity determinant.
Hedstrom L, Perona JJ, Rutter WJ. Hedstrom L, et al. Biochemistry. 1994 Jul 26;33(29):8757-63. doi: 10.1021/bi00195a017. Biochemistry. 1994. PMID: 8038165
However, chymotrypsin-like substrate specificity is attained when two surface loops are changed to the analogous residues of chymotrypsin, in conjunction with the changes in the S1 binding site [Hedstrom, L., Szilagyi, L., & Rutter, W. J. (1992) Science 2 …
However, chymotrypsin-like substrate specificity is attained when two surface loops are changed to the analogous residues of chymotrypsin, i …
172 results