Gräslund T - Search Results

1

Charge engineering of a protein domain to allow efficient ion-exchange recovery.

Gräslund T, Lundin G, Uhlén M, Nygren PA, Hober S. Gräslund T, et al. Protein Eng. 2000 Oct;13(10):703-9. doi: 10.1093/protein/13.10.703. Protein Eng. 2000. PMID: 11112509

2

Production of a thermostable DNA polymerase by site-specific cleavage of a heat-eluted affinity fusion protein.

Gräslund T, Nilsson J, Lindberg AM, Uhlén M, Nygren PA. Gräslund T, et al. Protein Expr Purif. 1997 Feb;9(1):125-32. doi: 10.1006/prep.1996.0674. Protein Expr Purif. 1997. PMID: 9116494

3

Display of active subtilisin 309 on phage: analysis of parameters influencing the selection of subtilisin variants with changed substrate specificity from libraries using phosphonylating inhibitors.

Legendre D, Laraki N, Gräslund T, Bjørnvad ME, Bouchet M, Nygren PA, Borchert TV, Fastrez J. Legendre D, et al. Among authors: graslund t. J Mol Biol. 2000 Feb 11;296(1):87-102. doi: 10.1006/jmbi.1999.3437. J Mol Biol. 2000. PMID: 10656819

4

In vitro selection of enzymatically active lipase variants from phage libraries using a mechanism-based inhibitor.

Danielsen S, Eklund M, Deussen HJ, Gräslund T, Nygren PA, Borchert TV. Danielsen S, et al. Among authors: graslund t. Gene. 2001 Jul 11;272(1-2):267-74. doi: 10.1016/s0378-1119(01)00527-3. Gene. 2001. PMID: 11470533

5

Strategy for highly selective ion-exchange capture using a charge-polarizing fusion partner.

Gräslund T, Ehn M, Lundin G, Hedhammar M, Uhlén M, Nygren PA, Hober S. Gräslund T, et al. J Chromatogr A. 2002 Jan 4;942(1-2):157-66. doi: 10.1016/s0021-9673(01)01413-3. J Chromatogr A. 2002. PMID: 11822381

6

Integrated strategy for selective expanded bed ion-exchange adsorption and site-specific protein processing using gene fusion technology.

Gräslund T, Hedhammar M, Uhlén M, Nygren PA, Hober S. Gräslund T, et al. J Biotechnol. 2002 Jun 13;96(1):93-102. doi: 10.1016/s0168-1656(02)00040-8. J Biotechnol. 2002. PMID: 12142146

7

Evaluation of different linker regions for multimerization and coupling chemistry for immobilization of a proteinaceous affinity ligand.

Linhult M, Gülich S, Gräslund T, Nygren PA, Hober S. Linhult M, et al. Among authors: graslund t. Protein Eng. 2003 Dec;16(12):1147-52. doi: 10.1093/protein/gzg121. Protein Eng. 2003. PMID: 14983098

8

Improving the tolerance of a protein a analogue to repeated alkaline exposures using a bypass mutagenesis approach.

Linhult M, Gülich S, Gräslund T, Simon A, Karlsson M, Sjöberg A, Nord K, Hober S. Linhult M, et al. Among authors: graslund t. Proteins. 2004 May 1;55(2):407-16. doi: 10.1002/prot.10616. Proteins. 2004. PMID: 15048831

9

Negatively charged purification tags for selective anion-exchange recovery.

Hedhammar M, Gräslund T, Uhlén M, Hober S. Hedhammar M, et al. Among authors: graslund t. Protein Eng Des Sel. 2004 Nov;17(11):779-86. doi: 10.1093/protein/gzh092. Epub 2004 Dec 3. Protein Eng Des Sel. 2004. PMID: 15579490

10

Single-step recovery and solid-phase refolding of inclusion body proteins using a polycationic purification tag.

Hedhammar M, Alm T, Gräslund T, Hober S. Hedhammar M, et al. Among authors: graslund t. Biotechnol J. 2006 Feb;1(2):187-96. doi: 10.1002/biot.200500023. Biotechnol J. 2006. PMID: 16892247

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