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Optimization of peptide linker length in production of MHC class II/peptide tetrameric complexes increases yield and stability, and allows identification of antigen-specific CD4+T cells in peripheral blood mononuclear cells.
Cunliffe SL, Wyer JR, Sutton JK, Lucas M, Harcourt G, Klenerman P, McMichael AJ, Kelleher AD. Cunliffe SL, et al. Eur J Immunol. 2002 Dec;32(12):3366-75. doi: 10.1002/1521-4141(200212)32:12<3366::AID-IMMU3366>3.0.CO;2-#. Eur J Immunol. 2002. PMID: 12432567 Free article.