Broos J - Search Results

1

Evaluation of the flow-dialysis technique for analysis of protein-ligand interactions: an experimental and a monte carlo study.

Veldhuis G, Vos EP, Broos J, Poolman B, Scheek RM. Veldhuis G, et al. Among authors: broos j. Biophys J. 2004 Apr;86(4):1959-68. doi: 10.1016/S0006-3495(04)74259-9. Biophys J. 2004. PMID: 15041640 Free PMC article.

2

Sensitive monitoring of the dynamics of a membrane-bound transport protein by tryptophan phosphorescence spectroscopy.

Broos J, Strambini GB, Gonnelli M, Vos EP, Koolhof M, Robillard GT. Broos J, et al. Biochemistry. 2000 Sep 5;39(35):10877-83. doi: 10.1021/bi000803z. Biochemistry. 2000. PMID: 10978174 Free article.

3

Mapping of the dimer interface of the Escherichia coli mannitol permease by cysteine cross-linking.

van Montfort BA, Schuurman-Wolters GK, Wind J, Broos J, Robillard GT, Poolman B. van Montfort BA, et al. Among authors: broos j. J Biol Chem. 2002 Apr 26;277(17):14717-23. doi: 10.1074/jbc.M201533200. Epub 2002 Feb 19. J Biol Chem. 2002. PMID: 11854301 Free article.

4

The first cytoplasmic loop of the mannitol permease from Escherichia coli is accessible for sulfhydryl reagents from the periplasmic side of the membrane.

Vervoort EB, Bultema JB, Schuurman-Wolters GK, Geertsma ER, Broos J, Poolman B. Vervoort EB, et al. Among authors: broos j. J Mol Biol. 2005 Feb 25;346(3):733-43. doi: 10.1016/j.jmb.2004.12.011. Epub 2005 Jan 11. J Mol Biol. 2005. PMID: 15713459 Free article.

5

Stoichiometry and substrate affinity of the mannitol transporter, EnzymeIImtl, from Escherichia coli.

Veldhuis G, Broos J, Poolman B, Scheek RM. Veldhuis G, et al. Among authors: broos j. Biophys J. 2005 Jul;89(1):201-10. doi: 10.1529/biophysj.105.062877. Epub 2005 May 6. Biophys J. 2005. PMID: 15879478 Free PMC article.

6

Substrate-induced conformational changes in the membrane-embedded IIC(mtl)-domain of the mannitol permease from Escherichia coli, EnzymeII(mtl), probed by tryptophan phosphorescence spectroscopy.

Veldhuis G, Gabellieri E, Vos EP, Poolman B, Strambini GB, Broos J. Veldhuis G, et al. Among authors: broos j. J Biol Chem. 2005 Oct 21;280(42):35148-56. doi: 10.1074/jbc.M507061200. Epub 2005 Aug 10. J Biol Chem. 2005. PMID: 16093245 Free article.

7

The oligomeric state and stability of the mannitol transporter, EnzymeII(mtl), from Escherichia coli: a fluorescence correlation spectroscopy study.

Veldhuis G, Hink M, Krasnikov V, van den Bogaart G, Hoeboer J, Visser AJ, Broos J, Poolman B. Veldhuis G, et al. Among authors: broos j. Protein Sci. 2006 Aug;15(8):1977-86. doi: 10.1110/ps.062113906. Epub 2006 Jul 5. Protein Sci. 2006. PMID: 16823033 Free PMC article.

8

Domain complementation studies reveal residues critical for the activity of the mannitol permease from Escherichia coli.

Vos EP, ter Horst R, Poolman B, Broos J. Vos EP, et al. Among authors: broos j. Biochim Biophys Acta. 2009 Feb;1788(2):581-6. doi: 10.1016/j.bbamem.2008.10.008. Epub 2008 Oct 29. Biochim Biophys Acta. 2009. PMID: 19013424 Free article.

9

Structure of the cytoplasmic loop between putative helices II and III of the mannitol permease of Escherichia coli: a tryptophan and 5-fluorotryptophan spectroscopy study.

Vos EP, Bokhove M, Hesp BH, Broos J. Vos EP, et al. Among authors: broos j. Biochemistry. 2009 Jun 16;48(23):5284-90. doi: 10.1021/bi8020668. Biochemistry. 2009. PMID: 19402710 Free article.

10

Localization of the substrate-binding site in the homodimeric mannitol transporter, EIImtl, of Escherichia coli.

Opacić M, Vos EP, Hesp BH, Broos J. Opacić M, et al. Among authors: broos j. J Biol Chem. 2010 Aug 13;285(33):25324-31. doi: 10.1074/jbc.M110.122523. Epub 2010 Jun 3. J Biol Chem. 2010. PMID: 20522557 Free PMC article.

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