A single amino acid substitution in the envelope protein of chimeric yellow fever-dengue 1 vaccine virus reduces neurovirulence for suckling mice and viremia/viscerotropism for monkeys.
Guirakhoo F, Zhang Z, Myers G, Johnson BW, Pugachev K, Nichols R, Brown N, Levenbook I, Draper K, Cyrek S, Lang J, Fournier C, Barrere B, Delagrave S, Monath TP.
Guirakhoo F, et al.
J Virol. 2004 Sep;78(18):9998-10008. doi: 10.1128/JVI.78.18.9998-10008.2004.
J Virol. 2004.
PMID: 15331733
Free PMC article.
During subsequent cell culture passaging of this clone for vaccine production, a single amino acid substitution (K to R) occurred in the envelope (E) protein at residue 204 (E204) (F. Guirakhoo, K. Pugachev, Z. Zhang, G. Myers, I. Levenbook, K. Draper, J. Lang, S. O …
During subsequent cell culture passaging of this clone for vaccine production, a single amino acid substitution (K to R) occurred in the env …