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Fermentation process for tetrameric human collagen prolyl 4-hydroxylase in Escherichia coli: improvement by gene optimisation of the PDI/beta subunit and repeated addition of the inducer anhydrotetracycline.
Neubauer A, Soini J, Bollok M, Zenker M, Sandqvist J, Myllyharju J, Neubauer P. Neubauer A, et al. Among authors: myllyharju j. J Biotechnol. 2007 Feb 1;128(2):308-21. doi: 10.1016/j.jbiotec.2006.10.017. Epub 2006 Nov 7. J Biotechnol. 2007. PMID: 17126943
Cytoplasmic production of an active human C-P4H has recently been described in the Origami (trxB gor) mutant Escherichia coli using a bicistronic vector with independent control of the alpha and PDI/beta subunit expression by the tetA and T5-lac promoters, respectively, enabling …
Cytoplasmic production of an active human C-P4H has recently been described in the Origami (trxB gor) mutant Escherichia coli using a bicist …
Wnt5a Deficiency Leads to Anomalies in Ureteric Tree Development, Tubular Epithelial Cell Organization and Basement Membrane Integrity Pointing to a Role in Kidney Collecting Duct Patterning.
Pietilä I, Prunskaite-Hyyryläinen R, Kaisto S, Tika E, van Eerde AM, Salo AM, Garma L, Miinalainen I, Feitz WF, Bongers EM, Juffer A, Knoers NV, Renkema KY, Myllyharju J, Vainio SJ. Pietilä I, et al. Among authors: myllyharju j. PLoS One. 2016 Jan 21;11(1):e0147171. doi: 10.1371/journal.pone.0147171. eCollection 2016. PLoS One. 2016. PMID: 26794322 Free PMC article.
149 results