Polyak SW - Search Results

1

Escherichia coli biotin protein ligase: characterization and development of a high-throughput assay.

Ng B, Polyak SW, Bird D, Bailey L, Wallace JC, Booker GW. Ng B, et al. Among authors: polyak sw. Anal Biochem. 2008 May 1;376(1):131-6. doi: 10.1016/j.ab.2008.01.026. Epub 2008 Jan 31. Anal Biochem. 2008. PMID: 18295588

2

Introduction of spacer peptides N-terminal to a cleavage recognition motif in recombinant fusion proteins can improve site-specific cleavage.

Polyak SW, Forsberg G, Forbes BE, McNeil KA, Aplin SE, Wallace JC. Polyak SW, et al. Protein Eng. 1997 Jun;10(6):615-9. doi: 10.1093/protein/10.6.615. Protein Eng. 1997. PMID: 9278273

3

Biotin protein ligase from Saccharomyces cerevisiae. The N-terminal domain is required for complete activity.

Polyak SW, Chapman-Smith A, Brautigan PJ, Wallace JC. Polyak SW, et al. J Biol Chem. 1999 Nov 12;274(46):32847-54. doi: 10.1074/jbc.274.46.32847. J Biol Chem. 1999. PMID: 10551847 Free article.

4

Mutational analysis of protein substrate presentation in the post-translational attachment of biotin to biotin domains.

Polyak SW, Chapman-Smith A, Mulhern TD, Cronan JE Jr, Wallace JC. Polyak SW, et al. J Biol Chem. 2001 Feb 2;276(5):3037-45. doi: 10.1074/jbc.M003968200. Epub 2000 Oct 19. J Biol Chem. 2001. PMID: 11042165 Free article.

5

Conserved Glu40 and Glu433 of the biotin carboxylase domain of yeast pyruvate carboxylase I isoenzyme are essential for the association of tetramers.

Jitrapakdee S, Surinya KH, Adina-Zada A, Polyak SW, Stojkoski C, Smyth R, Booker GW, Cleland WW, Attwood PV, Wallace JC. Jitrapakdee S, et al. Among authors: polyak sw. Int J Biochem Cell Biol. 2007;39(11):2120-34. doi: 10.1016/j.biocel.2007.06.006. Epub 2007 Jun 27. Int J Biochem Cell Biol. 2007. PMID: 17659996

6

Artifactual detection of biotin on histones by streptavidin.

Bailey LM, Ivanov RA, Wallace JC, Polyak SW. Bailey LM, et al. Among authors: polyak sw. Anal Biochem. 2008 Feb 1;373(1):71-7. doi: 10.1016/j.ab.2007.09.003. Epub 2007 Sep 8. Anal Biochem. 2008. PMID: 17920026

7

Reduced half-life of holocarboxylase synthetase from patients with severe multiple carboxylase deficiency.

Bailey LM, Ivanov RA, Jitrapakdee S, Wilson CJ, Wallace JC, Polyak SW. Bailey LM, et al. Among authors: polyak sw. Hum Mutat. 2008 Jun;29(6):E47-57. doi: 10.1002/humu.20766. Hum Mutat. 2008. PMID: 18429047

8

Microbial biotin protein ligases aid in understanding holocarboxylase synthetase deficiency.

Pendini NR, Bailey LM, Booker GW, Wilce MC, Wallace JC, Polyak SW. Pendini NR, et al. Among authors: polyak sw. Biochim Biophys Acta. 2008 Jul-Aug;1784(7-8):973-82. doi: 10.1016/j.bbapap.2008.03.011. Epub 2008 Apr 9. Biochim Biophys Acta. 2008. PMID: 18442489 Review.

9

Purification, crystallization and preliminary crystallographic analysis of biotin protein ligase from Staphylococcus aureus.

Pendini NR, Polyak SW, Booker GW, Wallace JC, Wilce MC. Pendini NR, et al. Among authors: polyak sw. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jun 1;64(Pt 6):520-3. doi: 10.1107/S1744309108012244. Epub 2008 May 23. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008. PMID: 18540065 Free PMC article.

10

Biotin protein ligase from Candida albicans: expression, purification and development of a novel assay.

Pendini NR, Bailey LM, Booker GW, Wilce MC, Wallace JC, Polyak SW. Pendini NR, et al. Among authors: polyak sw. Arch Biochem Biophys. 2008 Nov 15;479(2):163-9. doi: 10.1016/j.abb.2008.08.021. Epub 2008 Sep 11. Arch Biochem Biophys. 2008. PMID: 18809372

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