Willard FS - Search Results

1

Structural determinants of G-protein alpha subunit selectivity by regulator of G-protein signaling 2 (RGS2).

Kimple AJ, Soundararajan M, Hutsell SQ, Roos AK, Urban DJ, Setola V, Temple BR, Roth BL, Knapp S, Willard FS, Siderovski DP. Kimple AJ, et al. Among authors: willard fs. J Biol Chem. 2009 Jul 17;284(29):19402-11. doi: 10.1074/jbc.M109.024711. Epub 2009 May 28. J Biol Chem. 2009. PMID: 19478087 Free PMC article.

2

Established and emerging fluorescence-based assays for G-protein function: heterotrimeric G-protein alpha subunits and regulator of G-protein signaling (RGS) proteins.

Kimple RJ, Jones MB, Shutes A, Yerxa BR, Siderovski DP, Willard FS. Kimple RJ, et al. Among authors: willard fs. Comb Chem High Throughput Screen. 2003 Jun;6(4):399-407. doi: 10.2174/138620703106298491. Comb Chem High Throughput Screen. 2003. PMID: 12769684

3

Fluorescence-based assays for RGS box function.

Willard FS, Kimple RJ, Kimple AJ, Johnston CA, Siderovski DP. Willard FS, et al. Methods Enzymol. 2004;389:56-71. doi: 10.1016/S0076-6879(04)89004-9. Methods Enzymol. 2004. PMID: 15313559 Review.

4

Purification and in vitro functional analysis of the Arabidopsis thaliana regulator of G-protein signaling-1.

Willard FS, Siderovski DP. Willard FS, et al. Methods Enzymol. 2004;389:320-38. doi: 10.1016/S0076-6879(04)89019-0. Methods Enzymol. 2004. PMID: 15313574 Review.

5

A direct fluorescence-based assay for RGS domain GTPase accelerating activity.

Willard FS, Kimple AJ, Johnston CA, Siderovski DP. Willard FS, et al. Anal Biochem. 2005 May 15;340(2):341-51. doi: 10.1016/j.ab.2005.02.015. Anal Biochem. 2005. PMID: 15840508

6

The GAPs, GEFs, and GDIs of heterotrimeric G-protein alpha subunits.

Siderovski DP, Willard FS. Siderovski DP, et al. Among authors: willard fs. Int J Biol Sci. 2005;1(2):51-66. doi: 10.7150/ijbs.1.51. Epub 2005 Apr 1. Int J Biol Sci. 2005. PMID: 15951850 Free PMC article. Review.

7

The RGS protein inhibitor CCG-4986 is a covalent modifier of the RGS4 Galpha-interaction face.

Kimple AJ, Willard FS, Giguère PM, Johnston CA, Mocanu V, Siderovski DP. Kimple AJ, et al. Among authors: willard fs. Biochim Biophys Acta. 2007 Sep;1774(9):1213-20. doi: 10.1016/j.bbapap.2007.06.002. Epub 2007 Jun 29. Biochim Biophys Acta. 2007. PMID: 17660054 Free PMC article.

8

GTPase acceleration as the rate-limiting step in Arabidopsis G protein-coupled sugar signaling.

Johnston CA, Taylor JP, Gao Y, Kimple AJ, Grigston JC, Chen JG, Siderovski DP, Jones AM, Willard FS. Johnston CA, et al. Among authors: willard fs. Proc Natl Acad Sci U S A. 2007 Oct 30;104(44):17317-22. doi: 10.1073/pnas.0704751104. Epub 2007 Oct 19. Proc Natl Acad Sci U S A. 2007. PMID: 17951432 Free PMC article.

9

Structural determinants underlying the temperature-sensitive nature of a Galpha mutant in asymmetric cell division of Caenorhabditis elegans.

Johnston CA, Afshar K, Snyder JT, Tall GG, Gönczy P, Siderovski DP, Willard FS. Johnston CA, et al. Among authors: willard fs. J Biol Chem. 2008 Aug 1;283(31):21550-8. doi: 10.1074/jbc.M803023200. Epub 2008 Jun 2. J Biol Chem. 2008. PMID: 18519563 Free PMC article.

10

A point mutation to Galphai selectively blocks GoLoco motif binding: direct evidence for Galpha.GoLoco complexes in mitotic spindle dynamics.

Willard FS, Zheng Z, Guo J, Digby GJ, Kimple AJ, Conley JM, Johnston CA, Bosch D, Willard MD, Watts VJ, Lambert NA, Ikeda SR, Du Q, Siderovski DP. Willard FS, et al. Among authors: willard md. J Biol Chem. 2008 Dec 26;283(52):36698-710. doi: 10.1074/jbc.M804936200. Epub 2008 Nov 4. J Biol Chem. 2008. PMID: 18984596 Free PMC article.

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