Gene targeting using a promoterless gene trap vector ("targeted trapping") is an efficient method to mutate a large fraction of genes.
Friedel RH, Plump A, Lu X, Spilker K, Jolicoeur C, Wong K, Venkatesh TR, Yaron A, Hynes M, Chen B, Okada A, McConnell SK, Rayburn H, Tessier-Lavigne M.
Friedel RH, et al. Among authors: jolicoeur c.
Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13188-93. doi: 10.1073/pnas.0505474102. Epub 2005 Aug 29.
Proc Natl Acad Sci U S A. 2005.
PMID: 16129827
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