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Clinical manufacturing of recombinant human interleukin 15. I. Production cell line development and protein expression in E. coli with stop codon optimization.
Vyas VV, Esposito D, Sumpter TL, Broadt TL, Hartley J, Knapp GC 4th, Cheng W, Jiang MS, Roach JM, Yang X, Giardina SL, Mitra G, Yovandich JL, Creekmore SP, Waldmann TA, Zhu J. Vyas VV, et al. Among authors: knapp gc 4th. Biotechnol Prog. 2012 Mar-Apr;28(2):497-507. doi: 10.1002/btpr.746. Epub 2011 Dec 9. Biotechnol Prog. 2012. PMID: 22162520 Free PMC article.
Characterization of recombinant human IL-15 deamidation and its practical elimination through substitution of asparagine 77.
Nellis DF, Michiel DF, Jiang MS, Esposito D, Davis R, Jiang H, Korrell A, Knapp GC 4th, Lucernoni LE, Nelson RE, Pritt EM, Procter LV, Rogers M, Sumpter TL, Vyas VV, Waybright TJ, Yang X, Zheng AM, Yovandich JL, Gilly JA, Mitra G, Zhu J. Nellis DF, et al. Among authors: knapp gc 4th. Pharm Res. 2012 Mar;29(3):722-38. doi: 10.1007/s11095-011-0597-0. Epub 2011 Oct 19. Pharm Res. 2012. PMID: 22009587