Enhanced amino acid selection in fully evolved tryptophanyl-tRNA synthetase, relative to its urzyme, requires domain motion sensed by the D1 switch, a remote dynamic packing motif.
Weinreb V, Li L, Chandrasekaran SN, Koehl P, Delarue M, Carter CW Jr.
Weinreb V, et al. Among authors: li l.
J Biol Chem. 2014 Feb 14;289(7):4367-76. doi: 10.1074/jbc.M113.538660. Epub 2014 Jan 6.
J Biol Chem. 2014.
PMID: 24394410
Free PMC article.
We previously showed (Li, L., and Carter, C. W., Jr. (2013) J. Biol. Chem. 288, 34736-34745) that increased specificity for tryptophan versus tyrosine by contemporary Bacillus stearothermophilus tryptophanyl-tRNA synthetase (TrpRS) over that of TrpRS Urzyme results …
We previously showed (Li, L., and Carter, C. W., Jr. (2013) J. Biol. Chem. 288, 34736-34745) that increased specificity for tr …