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Precision-engineering the Pseudomonas aeruginosa genome with two-step allelic exchange.
Hmelo LR, Borlee BR, Almblad H, Love ME, Randall TE, Tseng BS, Lin C, Irie Y, Storek KM, Yang JJ, Siehnel RJ, Howell PL, Singh PK, Tolker-Nielsen T, Parsek MR, Schweizer HP, Harrison JJ. Hmelo LR, et al. Among authors: siehnel rj. Nat Protoc. 2015 Nov;10(11):1820-41. doi: 10.1038/nprot.2015.115. Epub 2015 Oct 22. Nat Protoc. 2015. PMID: 26492139 Free PMC article.
Bacterial fitness in chronic wounds appears to be mediated by the capacity for high-density growth, not virulence or biofilm functions.
Morgan SJ, Lippman SI, Bautista GE, Harrison JJ, Harding CL, Gallagher LA, Cheng AC, Siehnel R, Ravishankar S, Usui ML, Olerud JE, Fleckman P, Wolcott RD, Manoil C, Singh PK. Morgan SJ, et al. PLoS Pathog. 2019 Mar 20;15(3):e1007511. doi: 10.1371/journal.ppat.1007511. eCollection 2019 Mar. PLoS Pathog. 2019. PMID: 30893371 Free PMC article.
In Vivo Proteome of Pseudomonas aeruginosa in Airways of Cystic Fibrosis Patients.
Wu X, Siehnel RJ, Garudathri J, Staudinger BJ, Hisert KB, Ozer EA, Hauser AR, Eng JK, Manoil C, Singh PK, Bruce JE. Wu X, et al. Among authors: siehnel rj. J Proteome Res. 2019 Jun 7;18(6):2601-2612. doi: 10.1021/acs.jproteome.9b00122. Epub 2019 May 22. J Proteome Res. 2019. PMID: 31060355 Free PMC article.
Gallium disrupts bacterial iron metabolism and has therapeutic effects in mice and humans with lung infections.
Goss CH, Kaneko Y, Khuu L, Anderson GD, Ravishankar S, Aitken ML, Lechtzin N, Zhou G, Czyz DM, McLean K, Olakanmi O, Shuman HA, Teresi M, Wilhelm E, Caldwell E, Salipante SJ, Hornick DB, Siehnel RJ, Becker L, Britigan BE, Singh PK. Goss CH, et al. Among authors: siehnel rj. Sci Transl Med. 2018 Sep 26;10(460):eaat7520. doi: 10.1126/scitranslmed.aat7520. Sci Transl Med. 2018. PMID: 30257953 Free PMC article. Clinical Trial.
22 results